Additional Selective and Differential Cell Culture Media
NEOGEN Brilliant Green Bile 2% Broth, 500 g
Brilliant Green Bile Broth 2% is used for the detection of coliform bacteria in water, food, and dairy products in a laboratory setting. Brilliant Green Bile Broth 2% is not intended for use in the diagnosis of disease or other conditions in humans.The coliform group of bacteria includes aerobic and facultative anaerobic, Gram-negative, non-sporeforming bacilli that ferment lactose and form acid and gas at 35°C within 48 hours. Members of the Enterobacteriacae comprise the majority of this group, but organisms such as Aeromonas spp. may also be included. Procedures to detect and confirm coliforms are used in testing water, foods, dairy products and other materials. Brilliant Green Bile Broth 2% is used to confirm a positive presumptive test result.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-bile-2-percent-broth
NEOGEN Universal Pre-enrichment Broth, 500 g
Universal Pre-enrichment Broth is used for the recovery of Salmonella spp. and Listeria spp. in a laboratory setting. Universal Pre-enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans. Traditional methods for recovering Salmonella spp. and Listeria spp. from food products require separate pre-enrichment media for each microorganism. Some broth media recommended for preenrichment contain antibiotic inhibitors or have insufficient buffering capacity which hinder recovery of sublethally injured cells. Bailey and Cox formulated Universal Preenrichment Broth to permit simultaneous resuscitation of sublethally injured Salmonella and Listeria. The broth medium provides sufficient buffering capacity to prevent rapid decreases in pH and allows for repair of injured cells that might be sensitive to low pH values or inhibitory substances.For more information visit https://www.neogen.com/solutions/Microbiology/universal-preenrichment-broth
NEOGEN Buffered Peptone Water (BPW), 500 g
Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp. from food and is not intended for use in the diagnosis of disease or other conditions in humans. Edel and Kamelmacher found that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to Salmonella spp. Preenrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Lactose Broth is frequently used for this purpose, but it may be detrimental to recovering Salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods. For more information visit https://www.neogen.com/solutions/Microbiology/buffered-peptone-water
NEOGEN Listeria Enrichment Broth, 500 g
Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.Listeria monocytogenes, first described in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.For more information visit https://www.neogen.com/solutions/Microbiology/listeria-enrichment-broth
NEOGEN Modified Buffered Peptone Water with Pyruvate, 5 kg
Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans. Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables. Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-buffered-peptone-water-with-pyruvate
NEOGEN Brucella Agar, 5 kg
Brucella Agar is used for the cultivation of Brucella spp. and other fastidious microorganisms in a laboratory setting. Brucella Agar is not intended for use in the diagnosis of disease or other conditions in humans.Brucella Agar is prepared according to the APHA formula for Albimi Broth. Brucella Agar is a general-purpose medium for the cultivation of Brucella spp. and fastidious microorganisms including Streptococcus pneumoniae, Streptococcus viridans, and Neisseria meningitidis. With the addition of blood, Brucella Agar is used to determine bacterial hemolytic reactions. Brucella Agar can also be used as a base for the isolation of Campylobacter spp.For more information visit https://www.neogen.com/solutions/Microbiology/brucella-agar
NEOGEN KF Streptococcus Agar, 500 g
KF Streptococcus Agar is used with triphenyltetrazolium chloride for the selective isolation and enumeration of fecal streptococci in a laboratory setting. KF Streptococcus Agar is not intended for use in the diagnosis of disease or other conditions in humans.KF Streptococcus Agar was developed by Kenner, Clark, and Kabler for the detection of fecal streptococci in surface waters. KF Streptococcus Agar is currently used in detecting fecal streptococci in water, foods, and other materials through the membrane filtration technique or pour plate method. Fecal streptococci are normal inhabitants in the intestines, and the presence of fecal streptococci can be used as an indication of fecal contamination. The presence of fecal streptococci is of value in determining pollution sources because certain fecal streptococci are host-specific.For more information visit https://www.neogen.com/solutions/Microbiology/kf-streptococcus-agar
NEOGEN Brain Heart Infusion Agar, 500 g
Brain Heart Infusion Agar is used for the cultivation of a wide variety of fastidious organisms and is not intended for use in the diagnosis of disease or other conditions in humans.A general purpose nutritious agar base. This medium was first used for the isolation of dental pathogens. With the addition of 7% defibrinated blood the medium will support the growth of a wide range of fastidious organisms, the phosphate buffer will help neutralize the acids produced from the utilization of glucose and thus maintain viability. The medium is not recommended for the determination of hemolytic reactions because of the glucose content.
For more information visit https://www.neogen.com/solutions/Microbiology/brain-heart-infusion-agar
NEOGEN Bacillus cereus MYP Agar, 500 g
Bacillus Cereus MYP Agar is used with supplements for the selective and differential enumeration of Bacillus cereus from foods. Conforms to ISO and FDA / BAM formulation in a laboratory setting. MYP Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Formulated to ISO 7932:2004 MYP was introduced by Mossel and his co-workers in 1967 for the enumeration of Bacillus cereus in foods and was shown to be the most effective for this purpose by Inal in 1972. B. cereus is differentiated from other members of the Bacillus group by two reactions: mannitol fermentation and lecithinase production. Mannitol fermentation on this medium produces a yellow color. B. cereus is mannitol negative and produces red colonies. The lecithinase production of B. cereus is indicated by a white precipitate around the colonies.For more information visit https://www.neogen.com/solutions/Microbiology/bacillus-cereus-myp-agar
NEOGEN Brain Heart Infusion Broth, 500 g
Brain Heart Infusion Broth is used for the cultivation of a wide variety of fastidious organisms and is not intended for use in the diagnosis of disease or other conditions in humans. A rich isotonic infusion medium with tryptose (a mixture of meat and milk peptones) providing a wide range of substrates. A low concentration of glucose is used to stimulate early growth. The medium is lightly buffered to prevent the early death of some species due to acid production. Organisms which produce significant amounts of acid may well overwhelm the buffering system and auto-sterilize. The medium is suitable for use as a blood culture medium or as an enrichment broth for fastidious organisms. Formula:Brain Heart Infusion Solids (Porcine) - 17.5 g/L*Tryptose - 10.0 g/LGlucose - 2.0 g/LSodium Chloride - 5.0 g/LDisodium Hydrogen Phosphate - 2.5 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/brain-heart-infusion-broth
NEOGEN Harlequin E. coli/Coliform Agar, 500 g
E. coli / Coliform Agar is for the simultaneous enumeration of Escherichia coli and coliforms in food and environmental samples and is not intended for use in the diagnosis of disease or other conditions in humans.This dual chromogenic substrate medium makes the different colony types easy to distinguish allowing rapid counting of both E. coli and coliforms on a single agar.Based upon the formulation of Tryptone Bile Agar, the medium has been modified by the addition of two chromogenic substrates, one to detect the ß-glucuronidase enzyme (X- glucuronide) and another to detect the ß-galactosidase enzyme (magenta-ß-gal). Typical E. coli strains possess both enzymes but only cleave the X-glucuronide substrate, thereby producing blue-green colonies. Typical coliforms, however, possess only the ß-galactosidase enzyme and produce rose-pink colonies.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-e-coli-coliform-agar
NEOGEN Tryptic Soy Blood Agar Base No. 2, 500g
Tryptic Soy Blood Agar Base No. 2 is used with blood for the isolation and cultivation of a wide variety of microorganisms in a laboratory setting. Tryptic Soy Blood Agar Base No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.Blood Agar Bases are typically supplemented with 5 - 10% sheep, rabbit, or horse blood, and used for isolating, cultivating, and determining hemolytic reactions of fastidious pathogenic microorganisms. Without enrichment, this blood agar base can be used as a general purpose medium.Tryptic Soy Agar is based on the Soybean-Casein Digest formula specified by US Pharmacopeia. Tryptic Soy Agar is a nutritious base, and a variety of supplements, including blood, are often added to enhance this medium.For more information visit https://www.neogen.com/solutions/tryptic-soy-blood-agar-base-number-2
NEOGEN Brilliant Green Agar with Sulfapyridine, 500 g
Brilliant Green Agar w/ Sulfapyridine is used for the selective enrichment of Salmonella spp. in a laboratory setting. Brilliant Green Agar w/ Sulfapyridine is not intended for use in the diagnosis of disease or other conditions in humans.Brilliant Green Agar was first described by Kristensen et al and later modified by Kauffmann. The outstanding selectivity of this medium permits the use of moderately heavy inocula evenly distributed over the surface. The addition of sulfonamides into Brilliant Green Agar further inhibits Escherichia coli and Proteus spp. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar-with-sulfapyridine
NEOGEN Harlequin Chromogenic Agar for Salmonella Esterase (CASE), 500 g
CASE is a selective chromogenic agar for the detection of Salmonella that can be used as part of the ISO 6579-1:2017 workflow. It is not intended for use in the diagnosis of disease or other conditions in humans.CASE utilizes a dual chromogenic system to differentiate between Salmonella and non-target organisms that grow on the agar. The first chromogen is a target for esterase activity present in all Salmonella species, and results in blue/green colonies. The second chromogen is a target for ß-glucosidase activity, present in non-target organisms that are able to grow on the agar and also possess esterase activity. Utilization of the second chromogen masks the presentation of the first, resulting in black colonies in non-Salmonella organisms. All other organisms are either inhibited or grow colorless on the agar.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-chromogenic-agar-salmonella-esterase
NEOGEN Agar, Bacteriological No. 1, 500 g
Agar, Bacteriological No. 1 is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Bacteriological No. 1 is not intended for use in the diagnosis of disease or other conditions in humans. Agar, Bacteriological No. 1 conforms with the testing specified in the USP/EP Pharmacopeia.Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperature for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.For more information visit https://www.neogen.com/solutions/Microbiology/agar-bacteriological-number-1